产品介绍：本公司独家推出EASYspin无苯酚、氯仿RNA快速提取技术基础上，又独家研发成功基因组DNA清除柱技术确保有效清除gDNA残留，得到的RNA不需要DNase消化，可直接用于PCR、荧光定量PCR等实验。独特的裂解液/β-巯基乙醇迅速裂解细胞和灭活细胞RNA酶，植物RNA助提剂PLANTaid帮助结合多糖多酚并通过离心去除，然后裂解混合物通过一个基因组DNA清除柱，基因组DNA被清除而RNA穿透滤过。滤过的RNA用乙醇调节结合条件后,RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜， 再通过一系列快速的漂洗－离心的步骤, 去蛋白液和漂洗液将细胞代谢物，蛋白等杂质去除， 最后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。 产品特点：1. 完全不使用有毒的苯酚，氯仿等试剂，也不需要乙醇沉淀等步骤。2. 快速，简捷，单个样品操作一般可在30分钟内完成。3. 独有的植物RNA助提剂可以有效结合多糖多酚,提高清除效果。4. 独家研发成功基因组DNA清除柱技术确保有效清除gDNA残留，得到的RNA不需要DNase消化，可直接用于PCR、荧光定量PCR等实验。5. 多次柱漂洗确保高纯度，OD260/OD280典型的比值达1.9～2.0，基本无DNA残留,可用于RT-PCR，Northern-blot和各种实验。
RNA提取技术的领导者
RN09 EASYspin 植物RNA提取试剂盒
RN38 EASYspin Plus植物RNA提取试剂盒（带基因组清除柱）
RN40 EASYspin microRNA植物RNA提取试剂盒（世界独创，复杂植物microRNA也可提取)
特色
1. 操作简捷20-30分钟完成全部过程，简捷和速度第一。
2. 完全不用苯酚，氯仿抽提，安全环保第一。
3. 适应性广泛，可提取种类包括各种国产和进口公司提取失败复杂样品，如果实，种子，中草药，木本植物，多糖淀粉类，多酚类，色素类，可提取种类第一。
4. 可以用于包括转录组测序，荧光定量PCR等高标准应用。客户包括诺赛基因、华大基因、北京基因组研究中心、百迈克、等高通量公司。
5. 使用该系列产品发表文章59篇（仍旧在增加中），经验和可证实性第一。
6. 符合中国国情，技术指标远超Qiagen，价格仅为Qiagen的四分之一，性价比第一。
7. 中国创造已经申请专利，技术完胜Qiagen等进口公司产品。Qiagen提取失败的样品基本我们的全部可以提取。如果任何客户能找到Qiagen可以提取的植物样品，我们不能提取，每种样品奖励5000元！
简单选择指南：
1. 棉花，玉米，水稻，大豆，拟南芥，烟草等叶片类或者一般多糖多酚植物可以选择RN09。如果需要残留更少的DNA，可以选择增强版的RN38。如果需要下游做荧光定量PCR，可以配套DNA酶柱上消化试剂盒（RN3401) ，15分钟柱上直接消化残留DNA，洗脱下来RNA可以直接用于荧光定量PCR。
2. 特别复杂多糖多酚淀粉色素植物或者需要产量更高，如葡萄果实，草莓果实，人参根，水稻种子，小麦种子，蓝莓果实，松针，西瓜果实，香蕉果实等可以选择RN38（RN38配套有更强大裂解液选项）。如果从没有提取过的样品或者咨询我们也无法确定是否可以提取的样品，首选RN38，因为RN38一个盒子可以当RN09和RN38两种盒子使用，RN38是目前植物RNA提取试剂盒的集大成者，成功率基本为100%。
百合雌蕊 棉花叶片 榛子
玉米节间 琵琶果实 丹参microRNA
部分使用试剂盒发表的文章： 桃果实、花、根、叶：Isolation, characterisation and phylogenetic analysis of resistance gene analogues in a wild species of peach (Prunus kansuensis).Canadian Journal of Plant Science, 2011, 91(6): 961-970 樱桃花、叶、颚等各部位：Over-expression of the PaAP1 gene from sweet cherry (Prunus avium L.) causes early floweri.Journal of Plant Physiology, 2012,Available online 1 December 2012 洋葱根、茎、蕾、叶、雌雄蕊等各部位：Cloning and Expression Analysis of A Putative B Class MADS-box Gene of AcPI in Onion. Scientia Agricultura Sinica, 2012, 45(23):4759-4769 芜菁：Isolation and Functional Characterisation of the Genes Encoding Δ8-Sphingolipid Desaturase from Brassica rapa. Journal of Genetics and Genomics Volume 39, Issue 1, January 2012, Pages 47–59 芜 菁 1 ：EXPRESSION, DIVERGENCE AND EVOLUTION OF THE CALEOSIN GENE FAMILY IN BRASSICA RAPA. Arch. Biol. Sci., Belgrade, 65 (3), 863-876, 2013 DOI:10.2298/ABS1303863H 番茄叶：Effect of Low Temperature Stress on the Expression of ProDH Gene and the Activities of the Proline Dehydrogenase in Leaves of Tomato Seedling. Chinese Agricultural Science Bulletin 2012,28(10):132-135 栀子叶：Isolation of High Quality Total RNA from Gardenia jasminoides Eills.Chinese Agricultural Science Bulletin.2012, 28(27):194-198 油桐果实：Cui Qinqin, Han Xiaojiao, Chen Yicun, Zhan Zhiyong, Lin Liyuan, Wang Yangdong. Isolation and Expression Characteristics of Biotin Carboxyl Carrier Protein Coding Gene（VfBCCP) from Vernicia fordii.SCIENTIA SILVAE SINICAE. 2012, 48(8): Available online August 油桐果实1：Selection of Reliable Reference Genes for Gene Expression Studies Using Real-Time PCR in Tung Tree during Seed Development. PLoS ONE, 2012, 7(8): e43084 紫菜：Molecular cloning and expression analysis of ribosomal protein S7 gene from Porphyra haitanensis. JOURNAL OF FISHERIES OF CHINA, 2011, 35（12）：1814-1821 石斛：Molecular characterization of a mitogen-activated protein kinase gene DoMPK1 in Dendrobium officinale. Acta Pharmaceutica Sinica, 2012, 47 (12): 1703-1709 石斛1：ESTs Analysis Reveals Putative Genes Involved in Symbiotic Seed Germination in Dendrobium officinale. Symbiotic Germination Genes in D. officinale. August 2013 | Volume 8 | Issue 8 | e72705 大豆：RNA-seq Analysis Reveals Ethylene-Mediated Reproductive Organ Development and Abscission in Soybean(Glycine max L. Merr.). Plant Mol Biol Rep, 2012, published online: 4 Dec, 2012 大豆1：Construction of ethylene regulatory network based on the phytohormones related gene transcriptome profiling and prediction of transcription factor activities in soybean. Acta Physiol Plant, 2012, published online: 12 Dec, 2012 红花玉兰：Expression Analysis of MAwuAG in Different Organs and Developmental Stages of Magnolia wufengensis. Chinese Bulletin of Botany, 2013, 48 (2): 1–5 毛桃：Cloning and Phylogeny Analysis of PpAP2 Floral Homologous Genes in Peach. Chinese Agricultural Science Bulletin, 2013, 29(7): 99-104 五倍子：Cloning and characterisation of a phenylalanine ammonia-lyase gene from Rhus chinensis. Plant Cell Rep, 2013, published online：15 March, 2013 :五倍子1：Cloning, characterization and expression of chalcone synthase from medicinal plant Rhus chinensis.J. Plant Biochem. Biotechnol. DOI 10.1007/s13562-013-0231-9 青杄 ：cDNA Cloning and Bioinformatic Analysis of the sPPa1 Gene form Picea wilsonii. Plant Science Journal, 2012, 30(40): 394-401 青杄 1：cDNA Cloning and Bioinformatic Analysis of PsbO Gene from Picea wilsonii.Life Science Research, 2012, 16(3): 201-206 青杄 2：Cloning and Tissue Expression Analysis of PwPSAF in Picea wilsonii. SCIENTIA SILVAE SINICAE. Vol. 49，No. 10, Oct. 2013. 洋葱：Molecular Cloning and Transcriptional Analysis of the Putative AGAMOUS Homolog AcAG in Onion (Allium cepa. Plant Mol Biol Rep, DOI 10.1007/s11105-013-0607-y 木瓜：XsFAD2 gene encodes the enzyme responsible for the high linoleic acid content in oil accumulated in Xanthoceras sorbifolia seeds. JOURNAL ARTICLE. 2013-6-17. 木瓜1：Two novel diacylglycerol acyltransferase genes from Xanthoceras 2 sorbifolia are responsible for its seed oil content. GENE-38688; No. of pages: 9; 4C: 柑橘：Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection. Plant Cell Rep, DOI 10.1007/s00299-013-1470-x 柑橘1：Expression Analysis of Three Phloem-specific Promoters in Transgenic Poncirus trifoliata. Acta Horticulturae Sinica. 2014, 41(1): 1–8. 柑橘2： Activation of three pathogen-inducible promoters in transgenic citrus (Citrus sinensis Osbeck) after Xanthomonas axonopodis pv. citri infection and wounding. Plant Cell Tiss Organ Cult. DOI 10.1007/s11240-013-0423-y. 茶梅花瓣：Comparison and Analysis of Methods of Extracting Total RNA from Petals of Camellia sasanqua. Chinese Agricultural Science Bulletin.2013,29(28):129-133. 栀子：Isolation of High Quality Total RNA fromGardenia jasminoides Eills. Chinese Agricultural Science Bulletin. 2012, 28(27):194-198 丹参：Genome-wide analysis and molecular dissection of the SPL gene family in Salvia miltiorrhiza. 2014 Jan;56(1):38-50. doi: 10.1111/jipb.12111. Epub 2013 Nov 20. 牡丹：Transcriptome Comparison Reveals Key Candidate Genes Responsible for the Unusual Reblooming Trait in Tree Peonies. Genes Responsible for Reblooming in Tree Peonies. November 2013 | Volume 8 | Issue 11 | e79996 东南景天：Role of sulfur assimilation pathway in cadmium hyperaccumulation by Sedum alfredii Hance. Ecotoxicology and Environmental Safety. Volume 100, February 2014, Pages 159–165. 山苍子：Identification of appropriate reference genes for normalizing transcript expression by quantitative real‑time PCR in Litsea cubeba. TECHNICAL NOTE. Mol Genet Genomics (2013) 288:727–737, DOI 10.1007/s00438-013-0785-1 木本植物：Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants. ORIGINAL PAPER. Plant Cell Tiss Organ Cult, DOI 10.1007/s11240-013-0393-0 棉花：Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing. Sun et al. BMC Genomics 2013, 14:852 /1471-2164/14/852. 桃子：Biochemical changes and defence responses during the development of peach gummosis caused by Lasiodiplodia theobromae. Eur J Plant Pathol (2014) 138:195–207, DOI 10.1007/s10658-013-0322-4. 桃子1：Carbohydrate metabolism changes in Prunus persica gummosis infected with Lasiodiplodia theobromae. Phytopathology First Look paper • http://dx.doi.org/10.1094/PHYTO-01-13-0025-R • posted 11/27/2013. 海棠：The Malus crabapple transcription factor McMYB10 regulatesanthocyanin biosynthesis during petal coloration. Scientia Horticulturae 166 (2014) 42–49. 海藻：A rapid and sensitive method for field detection of Prorocentrum donghaiense using reverse transcription-coupled loop-mediated isothermal amplification. Harmful Algae 29 (2013) 31–39. 油茶：Establish a cDNA-AFLP Technology System in Camellia oleifera. Molecular Plant Breeding, 2013, Vol.11, No.5, 611-616. 亚洲百合：Transcriptomic analysis of Asiatic lily in the process of vernalization via RNA-seq. Mol Biol Rep. DOI 10.1007/s11033-014-3250-2. 毛泡桐：Dynamic expression of novel and conserved microRNAs and their targets in diploid and tetraploid of Paulownia tomentosa. Biochimie xxx (2014) 1e10. 人参：Cloning and Sequence Analysis Squalene Epoxidase Gene in Panax gin-seng. Journal of Jilin Agricultural University 2014, 36(2): 149-152,17 雪莲：Cloning and Sequence Analysis of rbcs Gene from Sasussured involucrdta Kar. et Kir. Chinese Agricultural Science Bulletin 2014, 30(15): 261-267 柑橘3：Secreted Expression of Cecropin B Gene Enhances Resistance to Xanthomonas axonopodis pv. citri in Transgenic Citrus sinensis‘Tarocco’ Acta Horticulturae Sinica 2014, 41(3): 417–428 http: // www. ahs. ac. cn 菊花：Stem apex detoxification culture markedly improved severalphysiological characters of chrysanthemum ‘YUTAI’. Plant Cell Tiss Organ Cult 2014, DOI 10.1007/s11240-014-0541-1 荞麦和拟南芥：Ectopic expression of FaesAP3, a Fagopyrum esculentum (Polygonaceae) AP3 orthologous gene rescues stamen development in an Arabidopsis ap3 mutant. Gene 2014, 550(2): 200–206 油松：Differential expression of SLOW WALKER2 homologue in ovules of female sterile mutant and fertile clone of Pinus tabulaeformis. Russian Journal of Developmental Biology 2014, 45(2): 78-84 玫瑰花：Precise spatio-temporal modulation of ACC synthase by MPK6 cascade mediates the response of rose flowers to rehydration. The Plant Journal 2014, 79(6): 941–950 棉花和拟南芥：Functional characterization of GhAKT1, a novel Shaker-like K+ channel gene involved in K+ uptake from cotton (Gossypium hirsutum). Gene 2014, 545(1): 61–71 棉花和拟南芥1：Upland Cotton Gene GhFPF1 Confers Promotion of Flowering Time and Shade-Avoidance Responses in Arabidopsis thaliana. PLoS ONE 2014, 9(3): e91869. doi:10.1371/journal.pone.0091869 白杨：Poplar GATA transcription factor PdGNC is capable of regulating chloroplast ultrastructure, photosynthesis, and vegetative growth in Arabidopsis under varying nitrogen levels. Plant Cell Tiss Organ Cult 2014, DOI 10.1007/s11240-014-0536-y 毛果杨：Molecular characterization of the SPL gene family in Populus trichocarpa. BMC Plant Biology 2014, 14: 131 葛根：Molecular cloning and characterization of an isoflavone 7-O-glucosyltransferase from Pueraria lobata. Plant Cell Reports 2014, 33(7), 1173–1185 百合：Cloning and Expression Analysis of Actin Gene（lilyActin）from Lily. Acta Horticulturae Sinica 2013, 40(7): 1318–1326 百合1： Vernalization of Oriental hybrid lily ‘Sorbonne’: changes in physiology metabolic activity and molecular mechanism. Molecular Biology Reports 2014, DOI 10.1007/s11033-014-3545-3 黄鹌菜：Transcriptome Sequencing and De Novo Analysis of Youngia japonica Using the Illumina Platform. PLoS ONE 2014, 9(3): e90636. doi:10.1371/journal.pone.0090636 棉花1：Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers. PLoS ONE 2014, 9(5): e96537. doi:10.1371/journal.pone.0096537 苹果：Low Medium pH Value Enhances Anthocyanin Accumulation in Malus Crabapple Leaves. PLoS ONE 2014, 9(6): e97904. doi:10.1371/journal.pone.0097904茶梅花瓣总RNA提取方法的比较和分析
吴田1，蓝增全2
（1西南林业大学园林学院，昆明650224；2西南林业大学环科学院，昆明650224）
摘要：总RNA的纯度和完整性对植物分子生物学实验至关重要，为了探索更适合茶梅花瓣总RNA的提取方法，本研究分别利用CTAB 多级沉淀法、改良的异硫氰酸胍法、改良的热硼酸法、Trizol 法和EASY spin 植物RNA提取试剂盒法等5 种方法提取茶梅花瓣总RNA，并进行了对比分析。结果发现艾德莱生物的EASY spin 植物RNA提取试剂盒法是最为简单、快速、高效的方法，该方法得到的RNA条带在琼脂糖凝胶上有清晰的28S、18S 和5S 3 条带，且超微量分光光度计测量的A260/280、A260/230均在允许范围内，表明该方法得到的RNA完整、质量高。CTAB多级沉淀法得到的RNA质量次之。本研究为今后有效开展茶梅分子生物学研究提供技术便利，并为其他花卉植物RNA的提取提供必要的参考。
本实验用了多种试剂提取RNA均没有得到较为理想的RNA 后，又多次尝试不同的RNA 试剂盒，如xxxxxxx此处省略N个公司N个产品，有兴趣可以看发表文章原文等，但均未能得到RNA，而EASYspin植物RNA提取试剂盒可以在较短的时间内获得高质量的RNA，说明该试剂盒中存在有效去除多糖物质的成分，而该成分是其他试剂盒中所缺少的。这也充分证明了植物材料RNA 提取的复杂性。综上所述，EASYspin 植物RNA提取试剂盒法提取方法简单、提取量大、RNA质量高，适合茶梅RNA的提取，充分显示了此方法在茶梅花瓣RNA提取上的优越性，因此，在实验经费允许的前提下，该方法是首选的，
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